Nanoethosomal Formulation of Gammaoryzanol for Skin-Aging Protection and Wrinkle Improvement: a histopathological study.

Nanoethosomal Formulation of Gammaoryzanol for Skin-Aging Protection and Wrinkle Improvement: a histopathological study.

Drug Dev Ind Pharm. 2017 Mar 03;:1-31

Authors: Heydari S, Ghanbarzadeh S, Anoosh B, Ranjkesh MR, Javadzadeh Y, Kouhsoltani M, Hamishehkar H

Abstract
BACKGROUND: Free radical scavengers and antioxidants, with the main focus on enhanced targeting to the skin layers, can provide protection against skin ageing.
OBJECTIVE: The aim of the present study was to prepare nanoethosomal formulation of Gammaoryzanol (GO), a water insoluble antioxidant, for its dermal delivery to prevent skin aging.
METHODS: Nanoethosomal formulation was prepared by a modified ethanol injection method and characterized by using laser light scattering, scanning electronic microscope (SEM) and X-ray diffraction (XRD) techniques. The effects of formulation parameters on nanoparticle size, encapsulation efficiency (EE%) and loading capacity (LC%) were investigated. Antioxidant activity of GO-loaded formulation was investigated in vitro using normal African green monkey kidney fibroblast cells (Vero). The effect of control and GO-loaded nanoethosomal formulation on superoxide dismutase (SOD) and Malondialdehyde (MDA) content of rat skin was also probed. Furthermore, the effect of GO-loaded nanoethosomes on skin wrinkle improvement was studied by dermoscopic and histological examination on healthy humans and UV-irradiated rats, respectively.
RESULTS: The optimized nanoethosomal formulation showed promising characteristics including narrow size distribution 0.17 ± 0.02, mean diameter of 98.9 ± 0.05 nm, EE% of 97.12 ± 3.62%, LC% of 13.87 ± 1.36% and zeta potential value of -15.1 ± 0.9 mV. The XRD results confirmed uniform drug dispersion in the nanoethosomes structure. In vitro and in vivo antioxidant studies confirmed the superior antioxidant effect of GO-loaded nanoethosomal formulation compared with control groups (blank nanoethosomes and GO suspension).
CONCLUSION: Nanoethosomes was a promising carrier for dermal delivery of GO and consequently had superior anti-aging effect.

PMID: 28277843 [PubMed - as supplied by publisher]

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