Fibroblast responses and antibacterial activity of Cu and Zn co-doped TiO2 for percutaneous implants

Publication date: 15 March 2018
Source:Applied Surface Science, Volume 434
Author(s): Lan Zhang, Jiaqi Guo, Ting Yan, Yong Han
In order to enhance skin integration and antibacterial activity of Ti percutaneous implants, microporous TiO2 coatings co-doped with different doses of Cu²⁺ and Zn²⁺ were directly fabricated on Ti via micro-arc oxidation (MAO). The structures of coatings were investigated; the behaviors of fibroblasts (L-929) as well as the response of Staphylococcus aureus (S. aureus) were evaluated. During the MAO process, a large number of micro-arc discharges forming on Ti performed as penetrating channels; O²⁻, Ca²⁺, Zn²⁺, Cu²⁺ and PO4³⁻ delivered via the channels, giving rise to the formation of doped TiO2. Surface characteristics including phase component, topography, surface roughness and wettability were almost the same for different coatings, whereas, the amount of Cu doped in TiO2 decreased with the increased Zn amount. Compared with Cu single-doped TiO2 (0.77Wt% Cu), the co-doped with appropriate amounts of Cu and Zn, for example, 0.55Wt% Cu and 2.53Wt% Zn, further improved proliferation of L-929, facilitated fibroblasts to switch to fibrotic phenotype, and enhanced synthesis of collagen I as well as the extracellular collagen secretion; the antibacterial properties including contact-killing and release-killing were also enhanced. By analyzing the relationship of Cu/Zn amount in TiO2 and the behaviors of L-929 and S. aureus, it can be deduced that when the doped Zn is in a low dose (<1.79Wt%), the behaviors of L-929 and S. aureus are sensitive to the reduced amount of Cu²⁺, whereas, Zn²⁺ plays a key role in accelerating fibroblast functions and reducing S. aureus when its dose obviously increases from 2.63 to 6.47Wt%.

Graphical abstract

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