The in vitro effect of Antimicrobial Photodynamic Therapy on dental microcosm biofilms from partially erupted permanent molars: a pilot study

Publication date: Available online 9 December 2017
Source:Photodiagnosis and Photodynamic Therapy
Author(s): Fabiana Sodré de Oliveira, Thiago Cruvinel, Daniela Alejandra Cusicanqui Méndez, Evandro José Dionísio, Daniel Rios, Maria Aparecida Andrade Moreira Machado
BackgroundThe aPDT could enhance the prevention of dental caries lesions in pits and fissures of partially erupted molars, by killing microorganisms from complex dental biofilms. This pilot study aimed to evaluate the effect of the Antimicrobial Photodynamic Therapy (aPDT) on the viability of specific microorganism groups of dental microcosm biofilms from occlusal surfaces of first permanent molars in eruption.MethodsDental microcosm biofilms grown on bovine enamel blocks, from dental plaque collected on occlusal surfaces of a partially erupted lower right first permanent molar, with McBain medium plus 1% sucrose in anaerobic condition at 37°C for 72h. The experiments were performed in eight groups: L-P-=no treatment (control), L18.75P-=18.75J/cm² LED, L37.5P-=37.5J/cm² LED, L75P-=75J/cm² LED, L-P+=200mM TBO, L18.75P+=200mM TBO+18.75J/cm² LED, L37.5P+=200mM TBO+37.5J/cm² LED, and L75P+=200mM TBO+75J/cm² LED. The counts of total microorganisms, total streptococci and mutans streptococci were determined on selective media agar plates by colony-forming units per mL. The log-transformed counts were analyzed by Kruskal-Wallis and post-hoc Dunn's test (P<0.05).ResultsThe counts of all microorganisms treated in the group L75P+ were statistically lower than those treated in L-P-. The aPDT promoted a significant reduction of microorganisms, with a trend of dose-dependent effect.ConclusionTBO-mediated aPDT was effective in reducing the viability of specific microbial groups in dental microcosm biofilms originated from occlusal of permanent molars in eruption.



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