Budding Yeast Has a Minimal Endomembrane System

Publication date: 8 January 2018
Source:Developmental Cell, Volume 44, Issue 1
Author(s): Kasey J. Day, Jason C. Casler, Benjamin S. Glick
The endomembrane system consists of the secretory and endocytic pathways, which communicate by transport to and from the trans-Golgi network (TGN). In mammalian cells, the endocytic pathway includes early, late, and recycling endosomes. In budding yeast, different types of endosomes have been described, but the organization of the endocytic pathway has remained unclear. We performed a spatial and temporal analysis of yeast endosomal markers and endocytic cargoes. Our results indicate that the yeast TGN also serves as an early and recycling endosome. In addition, as previously described, yeast contains a late or prevacuolar endosome (PVE). Endocytic cargoes localize to the TGN shortly after internalization, and manipulations that perturb export from the TGN can slow the passage of endocytic cargoes to the PVE. Yeast apparently lacks a distinct early endosome. Thus, yeast has a simple endocytic pathway that may reflect the ancestral organization of the endomembrane system.

Teaser

Although the mammalian endocytic pathway has early, late, and recycling endosomes, the Saccharomyces cerevisiae system is unresolved. Day et al. show that yeast only has two endosome types: the trans-Golgi network, functioning as both an early and recycling endosome, and the long-lived prevacuolar endosome, possibly reflecting the ancestral organization of the endomembrane system.


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