Making Anatomical Preparations of the Human Brain Using Colored Silicone for Vascular Perfusion Staining (Technical Description).

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Making Anatomical Preparations of the Human Brain Using Colored Silicone for Vascular Perfusion Staining (Technical Description).

World Neurosurg. 2018 Jan 22;:

Authors: Shkarubo MA, Shkarubo AN, Dobrovolsky GF, Polev GA, Chernov IV, Andreev DN, Karnaukhov VV, Koval KV

Abstract
INTRODUCTION: Thorough understanding of the central nervous system anatomy is a fundamental part of a neurosurgeon's training. Development of novel neurosurgical approaches and optimization of existing ones directly depend on the comprehensive study of topographic anatomy of the head and neck using cadaveric specimens. Staining the vessels of the arterial and venous systems of a cadaveric specimen aids the detailed understanding of the vascularization of anatomical structures of the brain.
PURPOSE: To present an alternative expedited method of making anatomical preparations of the human brain using colored silicone for vascular perfusion staining.
MATERIALS AND METHODS: A non-fixed, whole cadaver (with the head intact), without CNS pathology, no later than 48 hours after death, is used to prepare the human brain specimen through staining of arterial and venous vessels.
RESULTS: The proposed method provides a fast and inexpensive approach to making anatomical preparations of the human brain with staining of the vascular system. Obtained preparations can be used for educational purposes, aiding in the study of normal and topographic anatomy of the brain, as well as helping to develop new microsurgical and/or endoscopic approaches CONCLUSION: The proposed method allows, in experienced hands, to obtain such anatomical preparations relatively fast, at a low cost, and with excellent quality.

PMID: 29371167 [PubMed - as supplied by publisher]

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