A simple, inexpensive method for subcortical stereotactic targeting in nonhuman primates

Publication date: Available online 18 May 2018
Source:Journal of Neuroscience Methods
Author(s): J. Nicole Bentley, Siri S.S. Khalsa, Michael Kobylarek, Karen E. Schroeder, Kevin Chen, Ingrid L. Bergin, Derek M. Tat, Cynthia A. Chestek, Parag G. Patil
BackgroundMany current neuroscience studies in large animal models have focused on recordings from cortical structures. While sufficient for analyzing sensorimotor systems, many processes are modulated by subcortical nuclei. Large animal models, such as nonhuman primates (NHP), provide an optimal model for studying these circuits, but the ability to target subcortical structures has been hampered by lack of a straightforward approach to targeting.New MethodHere we present a method of subcortical targeting in NHP that uses MRI-compatible titanium screws as fiducials. The in vivo study used a cellular marker for histologic confirmation of accuracy.ResultsHistologic results are presented showing a cellular stem cell marker within targeted structures, with mean errors ± standard deviations (SD) of 1.40 ± 1.19 mm in the X-axis and 0.9 ± 0.97 mm in the Z-axis. The Y-axis errors ± SD ranged from 1.5 ± 0.43 to 4.2 ± 1.72 mm.Comparison with existing methodsThis method is easy and inexpensive, and requires no fabrication of equipment, keeping in mind the goal of optimizing a technique for implantation or injection into multiple interconnected areas.ConclusionThis procedure will enable primate researchers to target deep, subcortical structures more precisely in animals of varying ages and weights.



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