Development and characterization of a field-effect biosensor for the detection of acetoin

Publication date: 15 September 2018
Source:Biosensors and Bioelectronics, Volume 115
Author(s): Denise Molinnus, Lukas Muschallik, Laura Osorio Gonzalez, Johannes Bongaerts, Torsten Wagner, Thorsten Selmer, Petra Siegert, Michael Keusgen, Michael J. Schöning
A capacitive electrolyte-insulator-semiconductor (EIS) field-effect biosensor for acetoin detection has been presented for the first time. The EIS sensor consists of a layer structure of Al/p-Si/SiO2/Ta2O5/enzyme acetoin reductase. The enzyme, also referred to as butane-2,3-diol dehydrogenase from B. clausii DSM 8716^T, has been recently characterized. The enzyme catalyzes the (R)-specific reduction of racemic acetoin to (R,R)- and meso-butane-2,3-diol, respectively. Two different enzyme immobilization strategies (cross-linking by using glutaraldehyde and adsorption) have been studied. Typical biosensor parameters such as optimal pH working range, sensitivity, hysteresis, linear concentration range and long-term stability have been examined by means of constant-capacitance (ConCap) mode measurements. Furthermore, preliminary experiments have been successfully carried out for the detection of acetoin in diluted white wine samples.



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