Arsenic uptake, transformation, and release by three freshwater algae under conditions with and without growth stress

Abstract

This study was carried out using indoor controlled experiments to study the arsenic (As) uptake, biotransformation, and release behaviors of freshwater algae under growth stress. Three freshwater algae, Microcystis aeruginosa, Anabaena flosaquae, and Chlorella sp., were chosen. Two types of inhibitors, e.g., Cu²⁺ and isothiazolinone, were employed to inhibit the growth of the algae. The algae were cultivated to a logarithmic stage in growth media containing 0.1 mg/L P; then, 0.8 mg/L As in the form of arsenate (iAs^V) was added, while both inhibitors were simultaneously added at dosages of 0.1 and 0.3 mg/L, with no addition of inhibitors in the control. After 2 days of exposure, the average growth rate (μ_2d) was measured to represent the growth rates of the algae cells; the extra- and intracellular As concentrations in various forms, i.e., arsenate, arsenite (iAs^III), monomethyl arsenic (MMA), and dimethyl arsenic (DMA), were also measured. Without inhibitors, the average growth rate followed the order of M. aeruginosa, Chlorella sp., and A. flosaquae, with the growth rate of M. aeruginosa significantly higher than that of the other two algae. However, when Cu²⁺ was added as an external inhibitor, the order of the average growth rate for the three algae became partially reversed, suggesting differentiation of the algae in response to the inhibitor. This differentiation can be seen by the reduction in the average growth rate of M. aeruginosa, which was as high as 1730% at the 0.3-mg/L Cu²⁺ dosage when compared with the control, while for the other two algae, much fewer changes were seen. The great reduction in M. aeruginosa growth rate was accompanied by increases in extracellular iAs^V and iAs^III and intracellular iAs^V concentrations in the algae, indicating that As transformation is related to the growth of this algae. Much fewer or neglectable changes in growth were observed that were consistent with the few changes in the extra- and intracellular As speciation for the other two algae with Cu²⁺ inhibition and all the three algae with isothiazolinone inhibition, corroborating the above hypothesis again. All the algae tested in this study demonstrated great abilities for As transformation and release, as seen by the much higher rates of 86.11–99.98% and 81.11–99.89% for transformation and release when compared to the control, respectively. When inhibitors were added, the transformation and release values of only A. flosaquae decreased remarkably down to 72.37–86.79% and 64.67–85.24%, respectively, while no changes were seen for these values in the other two algae, indicating that growth stress did not affect the As transformation and release of the other algae. The biological productivity of As by the three algae followed the order of M. aeruginosa, Chlorella sp., and A. flosaquae, which was generally consistent with the As transformation and release in conditions with and without inhibitors, suggesting that the As behavior in the algae that was related to growth stress largely differed among algae species.

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