ALA-PDT suppressing the cell growth by Akt-/Erk-mTOR-p70 s6k pathway in human SZ95 sebocytes in vitro

Publication date: Available online 28 June 2018
Source:Photodiagnosis and Photodynamic Therapy
Author(s): Wenjie Liu, Qianqian Wang, Jiang Tuo, Yufeng Chang, Jiayi Ying, Min Jiang, Christos C. Zouboulis, Leihong Xiang
BackgroundTopical 5-aminolevulinic acid mediated photodynamic therapy (PDT) is known to be an effective method in treating acne vulgaris and other sebaceous gland-related diseases. The therapeutic mechanisms of ALA-PDT still remain undetermined. In this study, we aimed to investigate the upstream of mammalian target of rapamycin (mTOR) signaling cascade after ALA-PDT on cell growth of human SZ95 sebocytes.Material and methodsHuman SZ95 sebocytes were treated with different concentration of 5-ALA PDT. Western blotting was used to detect and analyze the protein expression level of P-Akt (T308)/Akt, P-Akt (S473)/Akt, P-Erk/Erk, P-AMPKα (T172)/AMPK, P-AMPKα1 (S485)/AMPKα2 (S491)/AMPK, P-PRAS40/PRAS40, RagC. Meanwhile, mTOR pathway activator IGF-1 and mTORC1 inhibitor rapamycin were added to observe the interferences of P-p70 S6K/p70 S6K after ALA-PDT.ResultsThe mTOR pathway inhibitor rapamycin enhanced the effect of ALA-PDT in SZ95 cells through decreasing the level of P-p70 s6k. Conversely, mTOR pathway activator IGF-1 reversed it. ALA-PDT reduced the level of P-Akt (T308), P-Erk, P-AMPKα (T172), P-AMPKα1 (S485)/AMPKα2 (S491) and P-PRAS40, and no change was observed in the level of Rag C.ConclusionALA-PDT suppresses the cell growth in SZ95 cells through Akt-/Erk- mTOR -p70 s6k pathway rather than PRAS40-/RagC- mTOR pathway.



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