Granulocyte Macrophage Colony-Stimulating Factor Alone Reduces Toxoplasma gondii Replication in Microglial Culture by Superoxide and Nitric Oxide, without IFN-γ production: A Preliminary Report

Publication date: Available online 7 June 2018
Source:Microbes and Infection
Author(s): Tamirys Simão Pimenta, Natalie Ferreira Chaves, Ana Paula Drummond Rodrigues, Cristovam Wanderley Picanço Diniz, Renato Augusto DaMatta, José Antônio Picanço Diniz Junior
In vitro studies have demonstrated that GM-CSF in combination with other stimulatory factors induces a microbicidal response that control T. gondii infection. We assessed whether GM-CSF alone can control T. gondiireplication in murine microglial cultures. Microglia were collected and cultured with or without GM-CSF and the half of each group was infected with T. gondii. We determined the T. gondii infectivity, cytokines levels, NO and superoxide detection.GM-CSF alone primes microglia, which after infection induces the production of TNF-α and IL-6, leading to NO and superoxide production, without any stimulus from IL-12p70 and IFN-γ.



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