Microbiologic assessment of flexible gastrointestinal endoscope reprocessing using a pump-assisted sampling technique: an investigation involving all endoscopy units in Tianjin, China

Publication date: July 2018
Source:American Journal of Infection Control, Volume 46, Issue 7
Author(s): Xue-Yue Ji, Pei-Yong Ning, Wei Zhang, Dong-Jing Yang, He Liu, Jun Liu, Jia Song, Chun-Nan Fei
BackgroundMicrobiologic surveillance of flexible gastrointestinal endoscopes is recommended in several guidelines as the primary means of identifying reprocessing failures. This study aimed to evaluate the contamination level and prevalence of bacteria of post-reprocessing endoscopes and to access whether using a pump-assisted sampling method (PASM) improves the sensitivity of culture.MethodsAll 59 endoscopy units in Tianjin, China, were investigated. The PASM and the conventional flushing sampling method (CFSM) were used to compare the results of the microbial culture. Logistic regression analysis was used to identify the influencing factors.ResultsOne hundred four (56.52%) flushing channel samples of gastrointestinal endoscopes were positive for culture, and the maximum bacterial concentration was 14,100 colony-forming units (CFU)/channel. One hundred fifty-one (82.07%) flushing samples were qualified according to the national standard of China (≤ 20 CFU/channel). The qualified rate of the samples collected by PASM was significantly lower than the qualified rate by CFSM (65.52% vs 89.68%). Using PASM (odds ratio [OR]: 4.257; 95% confidence interval [CI]: 1.870–9.690) would increase the sensitivity of culture. The use of purified water (OR: 0.288; 95% CI: 0.102–0.814) could reduce the risk of endoscope reprocessing failure.ConclusionMany endoscopes fail to meet the national standard for microbial culture after reprocessing. Our results suggest that using a pump-assisted method could increase the sensitivity of the test.



https://ift.tt/2tBZMox