Long Non-Coding RNA LINC0086 Functions as a Tumor Suppressor in Nasopharyngeal Carcinoma By Targeting miR-214.

Long Non-Coding RNA LINC0086 Functions as a Tumor Suppressor in Nasopharyngeal Carcinoma By Targeting miR-214.

Oncol Res. 2017 Feb 13;:

Authors: Guo J, Ma J, Zhao G, Li G, Fu Y, Luo Y

Abstract
Nasopharyngeal carcinoma (NPC) is a distinct head and neck cancer, occurring at high frequency in Southern China. Emerging studies have shown that long noncoding RNAs (lncRNAs) play critical roles in carcinogenesis and progression, including NPC. Here, we established a comprehensive lncRNA profiles in NPC and found that 35 lncRNAs were differentially expressed in NPC. We found that LINC0086 decreased in NPC patient serum samples and tissues. The Kaplan-Meier survival cure showed that the patients with high LINC0086 expression had higher survival rate than those with low LINC0086 expression. LINC0086 expression was associated with NPC histological grade, Lymph node metastasis and clinical stage. Upregulation of LINC0086 inhibited cancer cell proliferation and promoted apoptosis. In addition, Upregulation of LINC0086 dramatically decreased the expression of miR-214, an oncogene in several cancers, in C666-1 and HK-1 cells. And a miR-214 binding site was found in 3'UTR of LINC0086. We also validated that both miR-214 and LINC0086 presented in the RISC complex, demonstrating that LINC0086 could decrease miR-214 expression by directly interacting with miR-214. Furthermore, the suppressive effects of LINC0086 on NPC cell growth were reversed by overexpression miR-214 expression in vitro and in vivo. Thus, our study reports a novel mechanism underlying NPC carcinogenesis, and provides a potential novel diagnosis and treatment biomarker for NPC.

PMID: 28245169 [PubMed - as supplied by publisher]

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