Cultivation of Peptidiphaga gingivicola from subgingival plaque: the first representative of a novel genus of Actinomycetaceae

Summary

A novel bacterium was isolated from the subgingival plaque of a patient with periodontal disease. Bacterial strain BA112^T is a facultative gram-positive coccus. It metabolizes alanine, arginine, glycine, histidine, leucine, proline, serine, and tyrosine, but does not appear to utilize carbohydrates. Urease, esculin, indole, catalase, and nitrate reduction tests were all negative. Major cellular fatty acids were C_18:0, C_12:0, C_16:0, C_18:1w9c, and C_20:0. The genome was sequenced and is 2.4 Mbp in length and has 64% GC content. Based on phylogenetics of the 16S rRNA sequence and concatenated alignments of 37 conserved proteins, BA112^T belongs to the family Actinomycetaceae but is located on a branch of the tree without currently named members. Based on our phenotypic and phylogenetic studies, we propose that BA112^T is the first known representative of a new genus, for which the name Peptidiphaga gingivicola gen. nov., sp. nov. is proposed. The type strain is BA112^T.

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