Publication date: March 2018
Source:Journal of the Egyptian National Cancer Institute, Volume 30, Issue 1
Author(s): Arwa Farhat, Eiad Ali-Deeb, Amin Sulaiman, Majd Aljamali
Background and ObjectiveDevelopment of appropriate translational in vivo models is a prerequisite for personalized management of leukemic patients. Indeed, several immunodeficient mice models were developed for leukemias with main limitations due to their high cost, demanding management, and elongated assessment intervals. In this report, we aimed at evaluating the engraftment of CD34+ cells, isolated from an acute myeloid leukemia (AML) patient, in naturally immunodeficient chick embryo model.Methods and ResultsMononuclear cells or immunomagnetic sorted CD34+ cells were injected into chick embryo chorioallantoic membrane (CAM) veins. Seven days post-injection, human CD34 transcript was detected by reverse transcription polymerase chain reaction (RT-PCR) in blood, bone marrow (BM), spleen and liver from embryos injected with human leukemic cells. Interestingly, an amplicon of the same length has been detected in both BM and spleen from PBS injected embryos, although analysis via bioinformatics tools revealed no matches in chicken; neither in transcriptome nor in genome databases. Importantly, splenomegaly and hepatic lesions were observed in some CD34+ cells injected embryos.ConclusionCollectively, our data confirm the engraftment of primary human CD34+ leukemic cells in chick embryo liver, but other experiments are required to verify engraftment in BM and spleen, and to confirm the identity of a putative CD34 orthologous transcript in these two organs.
https://ift.tt/2GkLPmq
Σφακιανάκης Αλέξανδρος
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5 Άγιος Νικόλαος
Κρήτη 72100
00302841026182
00306932607174
alsfakia@gmail.com
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Reinforcing the utility of chick embryo model to in vivo evaluate engraftment of human leukemic stem cells
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- Acknowledgment to reviewers
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- An international comparison of risk factors betwee...
- Efficacy and safety of sublingual immunotherapy wi...
- Diagnostic criteria for acute food protein-induced...
- Haploidentical stem cell transplantation in a boy ...
- Small-airway dysfunction precedes the development ...
- Increase of natural killer cells in children with ...
- Exposure to dogs but not cats is associated to a d...
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- Immunomodulation of allergic response in children ...
- Fluticasone in mild to moderate atopic dermatitis ...
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