Transcription profile data of phorbol esters biosynthetic genes during developmental stages in Jatropha curcas

Publication date: June 2018
Source:Data in Brief, Volume 18
Author(s): Nurul Jadid, Rizal Kharisma Mardika, Kristanti Indah Purwani, Erlyta Vivi Permatasari, Indah Prasetyowati, Mohammad Isa Irawan
Jatropha curcas is currently known as an alternative source for biodiesel production. Beside its high free fatty acid content, J. curcas also contains typical diterpenoid-toxic compounds of Euphorbiaceae plant namely phorbol esters. This article present the transcription profile data of genes involved in the biosynthesis of phorbol esters at different developmental stages of leaves, fruit, and seed in Jatropha curcas. Transcriptional profiles were analyzed using reverse transcription-polymerase chain reaction (RT-PCR). We used two genes including GGPPS (Geranylgeranyl diphospate synthase), which is responsible for the formation of common diterpenoid precursor (GGPP) and CS (Casbene Synthase), which functions in the synthesis of casbene. Meanwhile, J. curcas Actin (ACT) was used as internal standard. We demonstrated dynamic of GGPPS and CS expression among different stage of development of leaves, fruit and seed in Jatropha.



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