NGS combined with phylogenetic analysis to detect HIV-1 dual infection in Romanian people who inject drugs

Publication date: Available online 4 April 2018
Source:Microbes and Infection
Author(s): Bogdan Popescu, Leontina Banica, Ionelia Nicolae, Eugen Radu, Iulia Niculescu, Adrian Abagiu, Dan Otelea, Simona Paraschiv
Dual HIV infections are possible and likely in people who inject drugs (PWID). Thirty-eight newly diagnosed patients, 19 PWID and 19 heterosexually HIV infected were analysed. V2-V3 loop of HIV-1 env gene was sequenced on the NGS platform 454 GSJunior (Roche). HIV-1 dual/multiple infections were identified in five PWID. For three of these patients, the reconstructed variants belonged to pure F1 subtype and CRF14_BG strains according to phylogenetic analysis. New recombinant forms between these parental strains were identified in two PWID samples. NGS data can provide, with the help of phylogenetic analysis, important insights about the intra-host sub-population structure.



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