Iodine-125 seed brachytherapy inhibits non–small cell lung cancer by suppressing epithelial-mesenchymal transition

Publication date: Available online 1 May 2018
Source:Brachytherapy
Author(s): Yana He, Liang Li, Jihua Liu, Xuening Zhang
PurposeMetastatic non–small cell lung cancer (NSCLC) has posed a great clinical challenge with high mortality. Iodine-125 (I-125) seed brachytherapy has not been widely applied in clinic against NSCLC.Methods and MaterialsMice were injected with human H23 NSCLC cells to establish a mouse xenograft model, which received I-125 seed implantation. The curative effect, pathological impairments, and survival rate of mice were investigated. Changes in the expression levels of epithelial-mesenchymal transition (EMT) markers, including N-cadherin, E-cadherin, and vimentin, in the xenograft tumors were analyzed using reverse transcription polymerase chain reaction and Western blot.ResultsThe tumor volume and pathological effect were reduced by I-125 seed implant. I-125 seed implant also significantly improved survival rate of the model mice. Expression patterns of N-cadherin, E-cadherin, and vimentin were reversed in I-125 seed–implanted mice in comparison with control mice, indicating suppressed EMT.ConclusionsI-125 seed brachytherapy significantly inhibits NSCLC by suppressing EMT in a mouse model.



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