Publication date: Available online 7 September 2017
Source:Molecular and Cellular Endocrinology
Author(s): Kiyotaka Itcho, Kenji Oki, Kazuhiro Kobuke, Yoko Yoshii, Haruya Ohno, Masayasu Yoneda, Noboru Hattori
This study aimed to evaluate the methylation levels of G protein-coupled receptor (GPCR) related genes and the effects of methylation on mRNA expression levels in aldosterone-producing adenoma (APA). DNA methylation array and transcriptome analysis were applied in non-functioning adrenocortical adenoma (NFA) and APA. We investigated 192 GPCR-related genes and found hypo-methylation in the promoter region of 66 of these genes in APA. An integration study between microarray and methylation analysis revealed that HTR4, MC2R, TACR1, GRM3, and PTGER1 showed hypo-methylation and up-regulation of mRNA in APA. qPCR analysis showed that HTR4 and PTGER1 expression was 9.3-fold and 6.6-fold higher in APAs than in NFAs, respectively, whereas expression of the other genes was not different between the groups. Methylation of HTR4 and PTGER1 at positions −229 and −666 from the transcription start site, respectively, showed a significant inverse correlation with their mRNA levels. Methylation levels were not associated with KCNJ5 or ATP1A1 mutations in human adrenal samples. We demonstrated an increased incidence of CpG island demethylation of GPCR-related gene in APA. The expression of two receptors, HTR4 and PTGER1, showed a strong association with DNA methylation.
http://ift.tt/2eN34Ry
Σφακιανάκης Αλέξανδρος
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5 Άγιος Νικόλαος
Κρήτη 72100
00302841026182
00306932607174
alsfakia@gmail.com
Αρχειοθήκη ιστολογίου
-
►
2023
(391)
- ► Φεβρουαρίου (200)
- ► Ιανουαρίου (191)
-
►
2022
(2843)
- ► Δεκεμβρίου (161)
- ► Σεπτεμβρίου (219)
- ► Φεβρουαρίου (264)
- ► Ιανουαρίου (280)
-
►
2021
(5625)
- ► Δεκεμβρίου (231)
- ► Σεπτεμβρίου (345)
- ► Φεβρουαρίου (620)
-
►
2020
(2065)
- ► Δεκεμβρίου (535)
- ► Σεπτεμβρίου (222)
- ► Φεβρουαρίου (28)
-
►
2019
(9608)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (3791)
- ► Ιανουαρίου (3737)
-
►
2018
(69720)
- ► Δεκεμβρίου (3507)
- ► Σεπτεμβρίου (3851)
- ► Φεβρουαρίου (8116)
- ► Ιανουαρίου (7758)
-
▼
2017
(111579)
- ► Δεκεμβρίου (7718)
-
▼
Σεπτεμβρίου
(7549)
-
▼
Σεπ 07
(347)
- Graphical Abstracts
- Meet Our Editorial Board Member
- Exercise Induced Neuroplasticity to Enhance Therap...
- Mitochondrial Permeability Transition Pore as a Su...
- Involvement of microRNA-146a in the Inflammatory R...
- Subacute Fluoxetine Reduces Signs of Hippocampal D...
- Ultra-micronized Palmitoylethanolamide: An Efficac...
- Demonstration of Biological and Immunological Equi...
- The Effect of Pyrroloquinoline Quinone on Apoptosi...
- Dendrites, 3rd Edition
- SAPHO syndrome associated with hidradenitis suppur...
- Teriflunomide-induced psoriasiform changes of fing...
- Evaluation of the relation between lipid coat, tra...
- Large-Scale Quantitative Proteomics Identifies the...
- Alternative Progenitor Cells Compensate to Rebuild...
- FPGA implementation of real-time SENSE reconstruct...
- Static and dynamic liver stiffness: An ex vivo por...
- MRI protocol optimization for quantitative DCE-MRI...
- The development and optimisation of 3D black-blood...
- COnstrained Data Extrapolation (CODE): A new appro...
- Reduced distortion artifact whole brain CBF mappin...
- Neonatal skin care: what should we do? A four-week...
- A photoconverter gel-assisted blue light therapy f...
- Triple cancers concurrently detected in a case of ...
- Histopathology of Urticaria
- Toxicity, uptake, and accumulation of nano and bul...
- EGFR T790M mutation testing of non-small cell lung...
- Histiocytoid cardiomyopathy and ventricular noncom...
- Upregulation of uc.189 in patients with esophageal...
- AEG-1 mRNA expression in non-small cell lung cance...
- Relationship between swallowing function and breat...
- Natural course of tonsillectomy pain: A prospectiv...
- Knowledge of food protein–induced enterocolitis sy...
- Lymphoproliferative responses to dendritic cell pr...
- Genetically modified products and food allergy
- Information for Readers
- The national cost of asthma among school-aged chil...
- Instructions for Authors
- Editorial Board
- The allergenicity of genetically modified foods fr...
- An unusual cause of fever in a patient with common...
- Penicillin skin testing
- Author's response
- Unusual presentation of combined immunodeficiency ...
- Table of Contents
- Predictors of granulomatous lymphocytic interstiti...
- Single recombinant and purified major allergens an...
- Sensitization profiles to peanut allergens across ...
- Allergic sensitization and objective measures of s...
- Evaluating proteins for potential allergenicity us...
- Knowledge of food protein–induced enterocolitis sy...
- Lymphoproliferative responses to dendritic cell pr...
- Genetically modified products and food allergy
- Information for Readers
- The national cost of asthma among school-aged chil...
- Instructions for Authors
- Editorial Board
- The allergenicity of genetically modified foods fr...
- An unusual cause of fever in a patient with common...
- Penicillin skin testing
- Author's response
- Unusual presentation of combined immunodeficiency ...
- Table of Contents
- Predictors of granulomatous lymphocytic interstiti...
- Single recombinant and purified major allergens an...
- Sensitization profiles to peanut allergens across ...
- Allergic sensitization and objective measures of s...
- Evaluating proteins for potential allergenicity us...
- Relationship between swallowing function and breat...
- Natural course of tonsillectomy pain: A prospectiv...
- Comparison of Localized and Systemic Otitis Media ...
- Assessing Readability and Reliability of Online Pa...
- Comparison of an Electromagnetic Middle Ear Implan...
- The TNF Receptor Superfamily-NF-κB Axis Is Critica...
- Leveraging blood and tissue CD4+ T cell heterogene...
- Novel mechanisms in immune tolerance to allergens ...
- Development of 1,3,4-oxadiazole thione based novel...
- Cardioprotective effect of Malva sylvestris L. in ...
- N(4)-[B-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan)me...
- Prophetic medicine as potential functional food el...
- Perillyl alcohol protects human renal tubular epit...
- An increased expression of long non-coding RNA PAN...
- Glycyrrhizic acid: A promising carrier material fo...
- MDR1 polymorphisms affect the outcome of Chinese m...
- Peroxisome proliferator-activated receptors as the...
- Antibody-cytokine fusion proteins for improving ef...
- Secondary Linburg-Comstock syndrome: a case report
- Cryopreserved Cadaveric Arterial Allograft for Art...
- Development of a standard reference material conta...
- River biofilm community changes related to pharmac...
- Letter to the editor: Response
- Endoscopic management for congenital pyriform sinu...
- The relationship between the GJB3 c.538C>T variant...
- Introducing nerve-sparing approach during minimall...
- Perioperative Therapies – Enhancing the Impact of ...
- Frequency and Prognostic Significance of Incidenta...
- Hostility and cognitive control: Evidence of incre...
- Role of B cells in T helper cell responses in a mo...
- Impaired Humoral and Cellular Immune Responses to ...
- Ormdl3 Upregulates Airway Smooth Muscle Proliferat...
-
▼
Σεπ 07
(347)
- ► Φεβρουαρίου (10753)
- ► Ιανουαρίου (10529)
-
►
2016
(16402)
- ► Δεκεμβρίου (7478)
- ► Φεβρουαρίου (900)
- ► Ιανουαρίου (1250)
! # Ola via Alexandros G.Sfakianakis on Inoreader
Η λίστα ιστολογίων μου
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Αρχειοθήκη ιστολογίου
-
►
2023
(391)
- ► Φεβρουαρίου (200)
- ► Ιανουαρίου (191)
-
►
2022
(2843)
- ► Δεκεμβρίου (161)
- ► Σεπτεμβρίου (219)
- ► Φεβρουαρίου (264)
- ► Ιανουαρίου (280)
-
►
2021
(5625)
- ► Δεκεμβρίου (231)
- ► Σεπτεμβρίου (345)
- ► Φεβρουαρίου (620)
-
►
2020
(2065)
- ► Δεκεμβρίου (535)
- ► Σεπτεμβρίου (222)
- ► Φεβρουαρίου (28)
-
►
2019
(9608)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (3791)
- ► Ιανουαρίου (3737)
-
►
2018
(69720)
- ► Δεκεμβρίου (3507)
- ► Σεπτεμβρίου (3851)
- ► Φεβρουαρίου (8116)
- ► Ιανουαρίου (7758)
-
▼
2017
(111579)
- ► Δεκεμβρίου (7718)
-
▼
Σεπτεμβρίου
(7549)
-
▼
Σεπ 07
(347)
- Graphical Abstracts
- Meet Our Editorial Board Member
- Exercise Induced Neuroplasticity to Enhance Therap...
- Mitochondrial Permeability Transition Pore as a Su...
- Involvement of microRNA-146a in the Inflammatory R...
- Subacute Fluoxetine Reduces Signs of Hippocampal D...
- Ultra-micronized Palmitoylethanolamide: An Efficac...
- Demonstration of Biological and Immunological Equi...
- The Effect of Pyrroloquinoline Quinone on Apoptosi...
- Dendrites, 3rd Edition
- SAPHO syndrome associated with hidradenitis suppur...
- Teriflunomide-induced psoriasiform changes of fing...
- Evaluation of the relation between lipid coat, tra...
- Large-Scale Quantitative Proteomics Identifies the...
- Alternative Progenitor Cells Compensate to Rebuild...
- FPGA implementation of real-time SENSE reconstruct...
- Static and dynamic liver stiffness: An ex vivo por...
- MRI protocol optimization for quantitative DCE-MRI...
- The development and optimisation of 3D black-blood...
- COnstrained Data Extrapolation (CODE): A new appro...
- Reduced distortion artifact whole brain CBF mappin...
- Neonatal skin care: what should we do? A four-week...
- A photoconverter gel-assisted blue light therapy f...
- Triple cancers concurrently detected in a case of ...
- Histopathology of Urticaria
- Toxicity, uptake, and accumulation of nano and bul...
- EGFR T790M mutation testing of non-small cell lung...
- Histiocytoid cardiomyopathy and ventricular noncom...
- Upregulation of uc.189 in patients with esophageal...
- AEG-1 mRNA expression in non-small cell lung cance...
- Relationship between swallowing function and breat...
- Natural course of tonsillectomy pain: A prospectiv...
- Knowledge of food protein–induced enterocolitis sy...
- Lymphoproliferative responses to dendritic cell pr...
- Genetically modified products and food allergy
- Information for Readers
- The national cost of asthma among school-aged chil...
- Instructions for Authors
- Editorial Board
- The allergenicity of genetically modified foods fr...
- An unusual cause of fever in a patient with common...
- Penicillin skin testing
- Author's response
- Unusual presentation of combined immunodeficiency ...
- Table of Contents
- Predictors of granulomatous lymphocytic interstiti...
- Single recombinant and purified major allergens an...
- Sensitization profiles to peanut allergens across ...
- Allergic sensitization and objective measures of s...
- Evaluating proteins for potential allergenicity us...
- Knowledge of food protein–induced enterocolitis sy...
- Lymphoproliferative responses to dendritic cell pr...
- Genetically modified products and food allergy
- Information for Readers
- The national cost of asthma among school-aged chil...
- Instructions for Authors
- Editorial Board
- The allergenicity of genetically modified foods fr...
- An unusual cause of fever in a patient with common...
- Penicillin skin testing
- Author's response
- Unusual presentation of combined immunodeficiency ...
- Table of Contents
- Predictors of granulomatous lymphocytic interstiti...
- Single recombinant and purified major allergens an...
- Sensitization profiles to peanut allergens across ...
- Allergic sensitization and objective measures of s...
- Evaluating proteins for potential allergenicity us...
- Relationship between swallowing function and breat...
- Natural course of tonsillectomy pain: A prospectiv...
- Comparison of Localized and Systemic Otitis Media ...
- Assessing Readability and Reliability of Online Pa...
- Comparison of an Electromagnetic Middle Ear Implan...
- The TNF Receptor Superfamily-NF-κB Axis Is Critica...
- Leveraging blood and tissue CD4+ T cell heterogene...
- Novel mechanisms in immune tolerance to allergens ...
- Development of 1,3,4-oxadiazole thione based novel...
- Cardioprotective effect of Malva sylvestris L. in ...
- N(4)-[B-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan)me...
- Prophetic medicine as potential functional food el...
- Perillyl alcohol protects human renal tubular epit...
- An increased expression of long non-coding RNA PAN...
- Glycyrrhizic acid: A promising carrier material fo...
- MDR1 polymorphisms affect the outcome of Chinese m...
- Peroxisome proliferator-activated receptors as the...
- Antibody-cytokine fusion proteins for improving ef...
- Secondary Linburg-Comstock syndrome: a case report
- Cryopreserved Cadaveric Arterial Allograft for Art...
- Development of a standard reference material conta...
- River biofilm community changes related to pharmac...
- Letter to the editor: Response
- Endoscopic management for congenital pyriform sinu...
- The relationship between the GJB3 c.538C>T variant...
- Introducing nerve-sparing approach during minimall...
- Perioperative Therapies – Enhancing the Impact of ...
- Frequency and Prognostic Significance of Incidenta...
- Hostility and cognitive control: Evidence of incre...
- Role of B cells in T helper cell responses in a mo...
- Impaired Humoral and Cellular Immune Responses to ...
- Ormdl3 Upregulates Airway Smooth Muscle Proliferat...
-
▼
Σεπ 07
(347)
- ► Φεβρουαρίου (10753)
- ► Ιανουαρίου (10529)
-
►
2016
(16402)
- ► Δεκεμβρίου (7478)
- ► Φεβρουαρίου (900)
- ► Ιανουαρίου (1250)
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου