Publication date: November 2017
Source:Journal of Dairy Science, Volume 100, Issue 11
Author(s): B.G. Carter, C.W. Park, M.A. Drake
Norbixin is the water-soluble carotenoid in annatto extracts used in the cheese industry to color Cheddar cheese. The purpose of norbixin is to provide cheese color, but norbixin is also present in the whey stream and contaminates dried dairy ingredients. Regulatory restrictions dictate that norbixin cannot be present in dairy ingredients destined for infant formula or ingredients entering different international markets. Thus, there is a need for the detection and quantification of norbixin at very low levels in dried dairy ingredients to confirm its absence. A rapid method for norbixin evaluation exists, but it does not have the sensitivity required to confirm norbixin absence at very low levels in compliance with existing regulations. The current method has a limit of detection of 2.7 μg/kg and a limit of quantification of 3.5 μg/kg. The purpose of this study was to develop a method to extract and concentrate norbixin for quantification in dried dairy ingredients below 1 μg/kg (1 ppb). A reverse-phase solid-phase extraction column step was applied in the new method to concentrate and quantify norbixin from liquid and dried WPC80 (whey protein concentrate with 80% protein), WPC34 (WPC, 34% protein), permeate, and lactose. Samples were evaluated by both methods for comparison. The established method was able to quantify norbixin in whey proteins and permeates (9.39 μg/kg to 2.35 mg/kg) but was unable to detect norbixin in suspect powdered lactose samples. The newly developed method had similar performance to the established method for whey proteins and permeates but was also able to detect norbixin in powdered lactose samples. The proposed method had a >90% recovery in lactose samples and a limit of detection of 28 ppt (ng/kg) and a limit of quantification of 94 ppt (ng/kg). The developed method provides detection and quantification of norbixin for dairy ingredients that have a concentration of <1 ppb.
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Σφακιανάκης Αλέξανδρος
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5 Άγιος Νικόλαος
Κρήτη 72100
00302841026182
00306932607174
alsfakia@gmail.com
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- Linear IgA bullous dermatosis associated with amyo...
- Milia: a useful clinical marker of CYLD mutation c...
- Scorpion bite-induced ischaemic stroke
- Oesophageal ischaemia: an uncommon cause of chest ...
- Posterior fossa progressive multifocal leukoenceph...
- Subclinical meningoventriculitis as a cause of obs...
- A unique case of nephrogenic systemic fibrosis fro...
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- Cryptococcus neoformans osteomyelitis and intramus...
- Olfactory bulb agenesis with normal sexual hormones
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- Syncope from radiation-induced baroreceptor failure
- Live 'Baby dance among the red blood cells
- Erdheim-Chester disease: atypical presentation of ...
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- Cancer Immunotherapy Trials Underutilize Immune Re...
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