HIV-1 Drug Resistance Profiles for the HIV Protease and Reverse transcriptase gene in patients Receiving Combination Therapy in Tehran, Iran.

HIV-1 Drug Resistance Profiles for the HIV Protease and Reverse transcriptase gene in patients Receiving Combination Therapy in Tehran, Iran.

Infect Disord Drug Targets. 2018 Apr 15;:

Authors: Sadeghi L, Lolaie M, Tabatabai RA, Bayanolhagh S, Taj L, Ahmadi NE, Abedinzadeh N, Vakili F, Ahmadi G, Mohraz M

Abstract
BACKGROUND: Determination of drug resistant mutations has a crucial role in the management of HIV-1 infected patients.
OBJECTIVE: The aim of the current study was to evaluate drug resistance profile of RT and PR gene, and find the correlation between drug resistance mutation and ART regimen to intensify physicians ' options for the most effective therapy and help to establish health related policies at the national level in Iran.
METHOD: HIV-1 RNA of 34 samples was extracted from plasma and RT Nested- PCR was performed and the final products were sequenced. Stanford HIV drug resistance sequence database was used for interpretation of the data.
RESULTS: In 14 patients out 15, the following mutations were observed; Nucleoside RT Inhibitor (NRTI)-Resistance mutations, with the highest frequency at codon 184 with the prevalence of 11(73%) patients and Non-Nucleoside RT Inhibitor (NNRTI)-Resistance Mutations, with the highest frequency at codon 103 with the prevalence of 8 (53%) patients .among 17 protease sequences, 2 (12%) major and 7 (41%) minor Protease Inhibitor (PI) Resistance Mutations were detected.
CONCLUSION: Having a high rate mutations in participants of this study raises concerns about treatment failure in HIV infected people in Iran.

PMID: 29663899 [PubMed - as supplied by publisher]

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