A pyrene-based colorimetric and fluorescent pH probe with large stokes shift and its application in bioimaging

Publication date: 1 November 2018

Source: Talanta, Volume 189

Author(s): Jianbin Chao, Kailun Song, Yongbin Zhang, Caixia Yin, Fangjun Huo, Juanjuan Wang, Ting Zhang

Abstract

A pyrene-based colorimetric and fluorescent pH probe with large Stokes shift (145 nm) was developed. PNY afforded a pK_a of 2.98 and responded linearly to minor pH changes within the range of 2.43 and 3.71. In addition, PNY could be used for detecting H⁺ with high sensitivity and selectivity, showing colorimetric and fluorometric dual-modal responses with short response time. Furthermore, PNY had good photostability, excellent reversibility and cell membrane permeability. Significantly, PNY could image acidic pH in A549 cells and visualized extreme acidity in E. coli cells.

Graphical abstract

Probe showed obvious color changes in the absorption and fluorescence titration. More importantly, Probe can be applied to detect the pH fluctuation in the E.coli cells.

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