Σφακιανάκης Αλέξανδρος
ΩτοΡινοΛαρυγγολόγος
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Δευτέρα 14 Σεπτεμβρίου 2020

Intestinal resection affects whole-body arginine synthesis

Intestinal resection affects whole-body arginine synthesis in neonatal piglets.:

Intestinal resection affects whole-body arginine synthesis in neonatal piglets.

Pediatr Res. 2020 Sep 13;:

Authors: Lansing M, Slim G, Wizzard P, Rafii M, Pencharz PB, Nation PN, Beggs MR, Todd Alexander R, Wales PW, Turner JM, Ball RO

Abstract

BACKGROUND: Previous studies in piglets show a direct relationship between intestinal mass and arginine (Arg) synthesis. We aimed to study the effects of 75% intestinal resection on whole-body Arg synthesis.

METHODS: Piglets were allocated to sham or jejunocolic (JC) surgery and to enteral nutrition (EN) at 20% [sham (n = 8), JC (n = 10)], or 40% [sham (n = 4), JC (n = 5)]. A gastric tube was placed for EN and a venous catheter for parenteral nutrition and blood sampling. On day 6, a primed bolus and constant infusion of Arg m + 2 label and proline m + 1 label was delivered. In addition, 40% EN piglets received a citrulline (Cit) m + 3 tracer. Blood sampling was undertaken and whole-body Arg synthesis was calculated. On day 7, intestinal length was measured, and samples were collected for gene expression (PCR quantification) and histopathology.

RESULTS: On Day 7, sham piglets showed intestinal lengthening compared to JC (p = 0.02). Whole-body Arg synthesis was similar between groups (p = 0.50). Adjusting for absolute small intestinal length, JC piglets had greater Arg synthesis (p = 0.01). Expression of arginosuccinase was upregulated in the jejunum of JC compared to sham on 20% EN (p = 0.03).

CONCLUSION: This demonstrates for the first-time adaptive changes in intestinal Arg synthesis following intestinal resection.

IMPACT: The intestine makes a critical contribution to whole-body arginine synthesis, particularly in neonates, a human population at risk for short bowel syndrome. Therefore, we studied intestinal arginine synthesis in a neonatal piglet model of short bowel syndrome and demonstrated adaptive changes in the intestine that may preserve whole-body arginine synthesis, despite loss of intestinal mass.This research adds new information to our understanding of the effects a massive intestinal resection has on amino acid metabolism during neonatal development.



PMID: 32920606 [PubMed - as supplied by publisher]

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