Publication date: Available online 1 October 2018
Source: Archives of Oral Biology
Author(s): Nayara Gonçalves Emerenciano, Alberto Carlos Botazzo Delbem, Juliano Pelim Pessan, Gabriel Pereira Nunes, Francisco Nunes Souza Neto, Emerson Rodrigues de Camargo, Marcelle Danelon
Abstract
Objective
To evaluate the effect of a fluoride toothpaste containing nano-sized sodium trimetaphosphate (TMPnano) on enamel demineralization in situ and composition of the biofilm.
Design
This crossover double-blind study consisted of four phases (seven days each) and 12 volunteers who wore oral appliances containing four enamel bovine blocks. The cariogenic challenge was performed by 30% sucrose solution (6x/day). The toothpaste treatments (3x/day) were as follows: no F/TMP/TMPnano (Placebo), 1,100 ppm F (1100 F), 1100 F plus 3% micrometric or nano-sized TMP (1100 F/TMP; 1100 F/TMPnano). Percentage of surface hardness loss (%SH), and integrated loss of subsurface hardness (ΔKHN), as well as enamel calcium (Ca), phosphorus (P), and fluoride (F) were determined. Moreover, biofilm formed on the blocks were analyzed for F, Ca, P, and insoluble extracellular polysaccharide (EPS) concentrations. Data were analyzed using one-way ANOVA, repeated measures followed by Fisher LSD test (p < 0.001).
Results
1100 F/TMPnano promoted the lowest %SH and ΔKHN among all groups (p < 0.001). Regarding the F concentrations in the enamel and in the biofilm, there were no significant differences between 1100 F and 1100 F/TMPnano, but significantly increased enamel Ca concentrations (p < 0.001). 1100 F/TMPnano showed lower values of EPS concentration when compared with 1100 F (~80%) (p < 0.001).
Conclusion
1100 F/TMPnano promoted a greater protective effect against enamel demineralization and significantly affected the composition of biofilm formed in situ when compared to 1100 F toothpaste.
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