Synthesis of chondroitin sulfate CC and DD tetrasaccharides and interactions with 2H6 and LY111

Publication date: Available online 3 February 2018
Source:Bioorganic & Medicinal Chemistry
Author(s): Kenya Matsushita, Tomomi Nakata, Naoko Takeda-Okuda, Satomi Nadanaka, Hiroshi Kitagawa, Jun-ichi Tamura
We synthesized the biotinylated chondroitin sulfate tetrasaccharides CS-CC [-3)βGalNAc6S(1–4)βGlcA(1-]2 and CS-DD [-3)βGalNAc6S(1–4)βGlcA2S(1-]2 which possess sulfate groups at O-6 of GalNAc and an additional sulfate group at O-2 of GlcA, respectively. We also analyzed interactions among CS-CC and CS-DD and the antibodies 2H6 and LY111, both of which are known to bind with CS-A, while CS-DD was shown for the first time to bind with both antibodies.

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